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Sequence Analysis of Hantaviruses Partial M Segment in the Suburbs of Qingdao

Author: LiZhenHai
Tutor: WangBin
School: Qingdao University
Course: Pathogen Biology
Keywords: Hantavirus M region genes Sequence analysis Gene tree
CLC: R373
Type: Master's thesis
Year: 2004
Downloads: 26
Quote: 0
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Abstract


The purpose of investigation from 2001 to 2003 in Qingdao, Shandong Province hantavirus infection and its molecular epidemiological characteristics of HTN and SEO type of epidemic strains of hantavirus in Qingdao, Shandong Province fast and accurate genotyping methods , the accumulation of epidemic learn information , and to lay the foundation for the rapid detection of diagnostic reagents and genotyping reagents and vaccine development . The method of collection HFRS acute period ( incidence of 1 to 5 days ) , 50 parts of serum samples extracted RNA as a template , according to the Gene Bank Find hantavirus - related sequence , the use of DNASTAR software design one pair of genus -specific primers material , the primer for HTN type and SEO - Universal cited material as the jacket PCR primer ; design of HTN -specific primer material and SEO -specific primer material as the inner sets of primers ; RT - Nest - PCR amplification of part of the hantavirus genome M fragment G1 gene area the nucleotide sequence of observations by UV transillumination . PCR products were inserted into the TA vector cloning and sequencing results of the sequencing analysis with DNASTAR software sequence , row , more dispersion . Results of HTN in 50 specimens with specific primers amplified 8 , accounting for 16% ; SEO type -specific primers amplified 29 , accounting for 58% ; 74% of the total positive rate . Overall , Qingdao the hantavirus strain of popular multi- SEO and HTN , SEO type . SEO Hantavirus relatively small differences in nucleotide sequence at the nucleotide sequence level , discrete gene was 0.3 to 8.9% . HTN Hantavirus high mutation rate , discrete gene was 1.8 to 13.1% . Conclusion Qingdao is a SEO type - based coexistence of HTN type of hantavirus the popular hybrid epidemic , which of HTN is mainly found in Jiaonan City . SEO Hantavirus is relatively conservative , relatively small differences in nucleotide sequence between the virus , HTN Hantavirus higher mutation rate .

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Human Virology ( pathogenic virus)
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