|
17beta-estradiol as a positive control, the use of life table and population accumulative culture methods, evaluation of the impact of aldrin and dieldrin calyciflorus rotifer reproduction. The main contents and results are as follows: 1, the use of life table of different concentrations (0.02,0.04,0.08,0.16,0.32,0.64,1.28 mg / L) Aldrin calyciflorus rotifers development, survival, and reproduction. The results showed that compared with the control group ,0.04-1 .28 mg / L of aldrin significantly shorten the rotifer embryonic development lasted; 0.16-1.28,0.04-0.16,0.02,0.08 and 0.16 mg / L of aldrin, respectively, significantly extend the the rotifer larval stages lasted the duration of reproductive period and the average life expectancy, and 1.28 mg / L aldrin rotifer significantly shorten the duration of reproductive period; 0.02-0.64,0.02-0.32,0.02,0.08 and 0.16 , 0.08 and 0.16 mg / L of aldrin were significantly improved or extend rotifer intrinsic rate of increase, net reproductive rate, life expectations and generation time, and 1.28 mg / L aldrin significantly shorten the rotifers The generation time. 2, the use of life table dieldrin different concentrations (0.001,0.01,0.1,1,10,100,1000 ug / L) and 17 beta-estradiol (E2) calyciflorus rotifers development, survival and reproduction. The results showed that compared with the control group, 0.01, 0.1 and 10 microg / L of dieldrin 0.001 to 0.1 and 10 microg / L E2 significantly shorten the rotifer embryonic development lasted; 0.01, 0.1 and 10 microg / L E2 significantly extend rotifer larval stage lasted, but 1000μg / L dieldrin significantly shorten the rotifer larval stages lasted; 0.001-100 ug / L dieldrin, 0.001,0.01,1,100 and 1000μg / L E2 significantly extended rotifer duration of reproductive period; 0.001 and 100 ug / L of dieldrin, 0.001,0.01,1,100 and 1000μg / L E2 significantly prolong the rotifer late reproductive lasted; 100 ug / L dieldrin significantly reduced rotifer intrinsic rate of increase, however 1μg / L of E2 significantly improve the the rotifer intrinsic rate of increase; 0.001μg / L dieldrin, 100 and 1000μg / L E2 significantly improves the the rotifers net reproductive rate; 0.001-1000μg / L dieldrin, 0.01,100 and 1000μg / L E2 was significantly longer generation time of rotifers; 0.001-1000μg / L of dieldrin, 0.01,1,100 In 1000μg / L E2 significantly extended life expectancy at hatching. 3, the use of experimental methods of population growth for three days and four days resting egg production of different concentrations (0.001,0.01,0.1,1,10,100,1000 ug / L) of aldrin, dieldrin and E2 on rotifer growth and sexual reproduction. 1000μg / L aldrin rotifer almost disappeared after 72 h. And compared to the control group, 0.01μg / L of E2, 0.001 and 0.1μg / L dieldrin agent significantly reduced the rotifer population growth rate, and 10 microg / L aldrin significantly improve the rotifer population growth rate; 1000μg / L of E2 concentration higher than 1μg / L of aldrin, the concentration is lower than 0.1 or higher than 10 microg / L of dieldrin significantly reduce the rate of rotifers mixed; 100 ug / L E2 1000μg / L dieldrin significantly reduced fertilization rate of rotifers; the 0.01 and 1000μg / L E2, 0.1μg / L aldrin, 0.001, 0.01, and 1000μg / L of dieldrin agent significantly reduce mixed females in the rotifer number / mixed females (MF / AF) ratio; aldrin concentration of more than 1μg / L, a concentration of 0.01 and 1000μg / L of dieldrin agent significantly reduced the wheel insect populations in the number of females with eggs / without eggs females (OF / NOF) the ratio of 0.1μg / L aldrin significantly improves the rotifers OF / NOF ratio. 1000μg / L E2, 100 ug / L aldrin, 0.001, 0.01, and 1000μg / L of dieldrin agent significantly reduces the rotifer resting egg production. 4, the use of groups, cumulative culture method (0.0002,0.002,0.02,0.2,2,20,200 ug / L) of different concentrations of aldrin and concentration of 0.001,0.01,0.1,1,10,100,1000 μg / L dieldrin and E2 on population growth. The results show that, compared with the control group, all concentrations of E2, 0.0002,0.02 and 0.2μg / L aldrin, 0.01,0.1,100 1000μg / L dieldrin agents significantly inhibited the rotifer population growth; 0.001 and 1000μg / L of E2 the 2μg / aldrin, 100 and 1000μg / L of dieldrin agent significantly reduced the maximum population density of rotifers.
|