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Hyaluronic acid not only from animal vitreous extract can also be obtained from the umbilical cord, skin, synovial fluid extraction. In addition, urine, plasma, skin thoracic lymph containing HA. HA is an indispensable part of the body, the body of HA content decreased with increasing age, especially skin tissue cells and reduce the moisture content, the gelatinous matrix mainly composed of HA cells filled space see small, resulting in tightly packed cells, collagen loca hardened, rough skin, loss of elasticity. Therefore, water is essential for skin health. Over the years, scientists have been of cosmetic moisturizing water as the main content of the study. The unique biological properties of hyaluronic acid and Hydrodynamics, determine its position in the cosmetics and pharmaceutical sectors. HA has a strong hydrophilic moisturizing capabilities and good biocompatibility, natural biomolecules is widespread in the skin and other tissues used in cosmetics from the 1980s, known as the ideal of \Moisturizing Factor. \Added HA cosmetics is called \Break through the traditional methods of the past hyaluronic acid extracted from biological tissue, and streptococcal strains from the bovine nasal screened production of hyaluronic acid, corn starch as raw materials, preparation of hyaluronic acid by fermentation means. Through the combination of physical and chemical means, mutagenic treatment of the wild-type strain, non-hemolytic, performance, stable production strains. Shake flask process conditions and to explore the use of the fermentation production of hyaluronic acid, batch fermentation process conditions, as well as the characteristics of batch fermentation kinetics. Improved batch fermentation using single fed-batch fermentation, the enormous increase in the yield of HA. Investigated extraction of hyaluronic acid in the fermentation broth extraction conditions on the extraction yield. This topic is part of the key technology development projects in Jilin Province in 2000 the use of corn starch as raw material hyaluronic acid \This thesis is focused on the following key technologies. Determine the effective strain screening and mutagenesis. Take the nasal mucosa of freshly killed cattle, cultured diluted lt; WP = 124 gt; were cultured on blood agar medium, cultivate the end, by the judgment of microbial bacteria in blood agar colonies and hemolytic characteristics, to select the initial screening strains. Re-screened, and the product has a typical HA infrared absorption characteristics, and to this strain to determine the starting strain to produce hyaluronic acid. UV and nitrosoguanidine mutagenesis, to get stable performance, no hemolytic mutant. Determine the mutant facilitate the dyeing method and preservation method. To optimize the composition of the seed medium, determined with a carbon source, a nitrogen source, metal ions, etc. The amount of 2% starch, glucose hydrolyzate can be used as a carbon source, the use of 2% peptone and yeast powder a mixture of an organic nitrogen source and 0.4% nitrite is an inorganic nitrogen source, MgSO4 · 7H2O 0.1%, KH2PO4 0.2%, and carbonitrides of a 1:1 ratio of medium as a seed medium, is conducive to the growth of this strain. Orthogonal optimization experimental design methods to obtain a shake flask fermentation medium composition: 4% of glucose starch hydrolyzate, 2% peptone and yeast powder a mixture of an organic nitrogen source and 0.5% of sodium nitrite is an inorganic nitrogen source, MgSO4 · 7H2O 0.1%, KH2PO4 0.2%. Of glucose using starch hydrolyzate as carbon source and peptone and yeast extract the complex nitrogen source the main nutritional composition of the medium, instead of fetal calf serum medium, greatly reducing the fermentation cost herein mutant strains obtained by mutagenesis in adequate and inadequate oxygen supply conditions can a lot of growth, synthesis of hyaluronic acid. However, good ventilation, HA yield higher. So shake flask culture, 500mL triangular flask liquid volume of 20mL, maximum yield obtainable. Using 5L small fermentation tank, batch fermentation of the mutant strains obtained by mutagenesis after the culture conditions of the production of HA. Were investigated the initial glucose concentration, pH value, the culture temperature, oxygen transfer, adding the synthesis of HA precursors on fermentation. The results show that the high initial sugar concentration on cell growth and HA synthesis inhibition, found that 5% of the initial sugar concentration is more appropriate. The ideal small can batch fermentation conditions: inoculum age of 14-16h, stirring speed of 200rpm, the pH value of 7.0, incubation temperature 37 ° C, ventilation 2L/L.min. HA synthesis precursor UMP may be added to promote bacterial reproduction and greatly increase the HA production. The papers on the breeding of strains using starch the glucose hydrolyzate fermentation production of hyaluronic acid fermentation process, based on statistical thermodynamics, combined with some classic method of microbial kinetic models to describe the entire growth of the fermentation process, product formation and substrate consumption dynamics model, and experiments to determine the model parameters. Use of the mathematical model and the experimental data, the calculated and experimental lt; WP = 125 gt; values ??fit well, indicating that the model can be used to describe the strain under normal conditions glucose using starch hydrolysis liquid fermentation production of hyaluronic acid production process. Because HA fermentation has while suppressing the dynamic characteristics of the substrate and product. The HA high levels of fermentation by feeding. This study used a single fed-batch culture method and the batch fermentation, the cell growth in the fermentation process, the product synthesis and byproducts of view, the method can be to improve the environmental conditions, to improve the fermentation process The yield of HA. And compared to batch fermentation, the fermentation broth the HA amount of about 3.5%. The HA fermentation using single fed-batch fermentation method to increase the intensity of production, shorten the fermentation cycle, reduce production costs, an application effectively to improve the the HA fermentation performance of modern fermentation technology. Extracted cosmetic grade hyaluronic acid, white refined extract HA glucuronic acid content of 31.3%, specific rotation [α] 20D = -68 °. But hyaluronic lower yield to give a pale yellow First extract HA 2.77g / L, the white refined extract HA 0.98g / l. This paper examines the impact of extraction conditions on the extraction rate, that uses the temperature to 30 ° C, pH 7 extraction conditions to extract hyaluronic acid in the fermentation broth is more appropriate, determined using a combination of ethanol and CPC approach to extract The method of HA. Standard and the present study of the hyaluronic acid in the fermentation broth extract using an infrared spectrophotometer analysis, because
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