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Detection and Significance of Inhibitor of Differentiation-1 in Cervical Cancer

Author: QuLiPing
Tutor: YuXiaoLing
School: Qingdao University
Course: Pathology and Pathophysiology
Keywords: Cervical neoplasms Id-1 Real time PCR immunohistochemistry
CLC: R737.33
Type: Master's thesis
Year: 2011
Downloads: 12
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Abstract


Objective:To detect the mRNA and protein expression of Id-1 (inhibitor of differentiation or inhibitor of DNA binding) in human cervical cancer and analysis its clinical significance.Methods:The quantity of Id-1 mRNA was detected by real time reverse transcription-polymerase chain reaction (real time RT-PCR), and the protein expression was detected by immunohistochemical method in 6 normal cervical samples,6 cervical intraepithelial neoplasias(CIN) and 24 cervical cancers. The relationship between Id-1 and some clinical parameters was further analyzed.Results:No Id-1 mRNA and protein was shown in normal cervical tissues. The quantity of Id-1 mRNA was obviously difference in normal,CIN and cervical cancers(F=3030.30, q=17.78～95.04, P<0.01). Higher expression of Id-1 protein was shown in cervical cancers than that in CIN (t=7.80, P<0.01 and t=3.15, P<0.01).The expression of Id-1 mRNA and protein was positive correlation in cervical cancers (r=0.89, P<0.01). Moreover, higher expression of Id-1 was correlated with the differentiation and FIGO stage in ovarian cancers (r=0.67,P<0.01 and r=0.58,P<0.05)Conclusion:The high expression of Id-1 is obviously correlated with the pathological grade and clinical stage in cervical cancer. Detection of Id-1 can help the diagnosis and prognosis of cervical cancer.

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