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The purpose of ischemic stroke is one of the three diseases to the human toll, that the incidence of ischemic stroke is determined by a variety of factors, genetic factors play a decisive role in the susceptibility to ischemic stroke related gene polymorphisms and gene mutation studies on the potential effects of ischemic stroke ischemic stroke research hotspot. Susceptibility gene polymorphism, will contribute to the understanding of the pathogenesis of ischemic stroke, and is expected to open up new avenues for the thorough treatment of ischemic stroke from the molecular level from the molecular level. Atherosclerosis is the underlying cause of ischemic stroke, the inflammatory response is a key link in the development of atherosclerotic disease, on endothelial cell adhesion and extravascular migration of inflammatory cells in the circulating inflammatory response from start link. Platelet endothelial cell adhesion molecule -1 (platelet endothelial cell adhesion molecule-1, PECAM-1) is a necessary condition of leukocyte adhesion in endothelial transmembrane transport, to the extravascular migration. In this study, platelet endothelial cell adhesion molecule -1 (PECAM-1) the gene Leu125Val Asn563Ser sites gene polymorphism and the population of North China (the Liaoning region) of ischemic stroke relationship. Method 1. 169 patients with ischemic stroke patients (case group) and age, 186 cases of sex-matched normal subjects (control group), determination of ischemic stroke clinical indicators, including blood lipids, blood sugar, high blood pressure, BMI. The subjects fasted for 12 hours, the patient was admitted to hospital the next morning, medical examination the day to extract peripheral venous blood 2 ml of EDTA anticoagulant. Genomic DNA was extracted using DNA extraction kit, and for DNA quantification and purity analysis. 4. Use premier5.0 design primers by biotech companies preparation primer. 5. Detected by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method PEC AM-1 genotype. The PCR product was directly sequenced to verify the results of PCR-RFLP. This step is completed by Shanghai Sangon Biological Engineering Technology \u0026 Services Co., Ltd.. Were compared genotype and allele frequency distribution and relationship with ischemic stroke. And analysis of the clinical data and experimental indicators between the two groups. Results 1. Ischemic stroke group PECAM-1 L125V locus LL LV VV genotype frequencies were: 17.2%, 51.5%, 31.3%, and the control group were 23.1%, 56.5%, 20.4%, gene type frequency in accordance with the Hardy-Weinberg equilibrium, the case group and the control group of PECAM-1 LI25V genotype distribution in comparison with a statistically significant difference (X2 = 6.082, P = 0.048). Polymorphism loci of PECAM-1S563A AA, AS, SS genotype frequency of 29.0%, 51.5% and 19.5%, respectively. The control group were 23.7%, 53.2%, 23.1%. The genotype frequencies in Hardy-Weinberg equilibrium. Case and control groups PECAM-1 S563A genotype distribution does not have a statistically significant (X2 = 1.385, p = 0.5). Ischemic stroke group PECAM-1 L125V V allele frequency was 57.1% in the control group was 48.7%, V allele differences in the distribution of ischemic stroke and control groups was significant (X2 = 5.066, p = 0.024). Of PECAM-case group the S563A A allele frequency was 54.7%, the A allele frequency of the control group was 50.3%. Allele frequency nor the case group and the control group was statistically significant (X2 = 1.258, p = 0.262). 3.Logistic regression analysis results show that the PECAM-1L125V polymorphic loci VV genotype is an independent risk factor for ischemic stroke (OR = 2.467,95% CI :1.518-4 .009, p = 0.000). Conclusion of PECAM-1 gene Leu125Val sites of gene polymorphism related to ischemic stroke, VV genotype may be a genetic susceptibility factors for ischemic stroke population of North China.
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