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Objective: ovarian cancer is one of the female genital three malignant tumors, due to the incidence of occult, the lack of specificity of early indications of disease, often diagnosed had transferred about 30% of the 5-year survival rate of patients with ovarian cancer , the mortality rate ranks first in the gynecological malignancies. Although so far the etiology and pathogenesis of ovarian cancer is not clear, but some research suggests, angiogenesis, development and infiltration of human solid tumors, including ovarian cancer, including transfer plays an important role. Known angiogenic factor, vascular endothelial growth factor (vascular endothelial growth factor, VEGF) is a specific, strong vascular endothelial cell mitogenic factor and vascular endothelial growth factor, tumor growth, invasion and metastasis Relevance. Human VEGF gene promoter region, there are some polymorphic loci polymorphic loci may be affected by changing the transcriptional activity of the gene expression of the protein, resulting in individual differences in human susceptibility to cancer. The purpose of this study is to investigate the promoter region of the VEGF gene-1154G / A,-460C / T single nucleotide polymorphisms (single nucleotide polymorphism, SNP) and the risk of epithelial ovarian cancer, from the molecular biology of ovarian provide the basis for prevention, diagnosis and treatment of cancer. Methods: A case - control study, collected 303 cases of epithelial ovarian cancer patients and 303 healthy control individuals intravenous anticoagulant each 5mL recorded history and personal and family information. Proteinase K digestion - salting out method extracted from peripheral blood DNA by polymerase chain reaction - restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP) analysis was used to detect VEGF gene promoter region-1154G / A,-460C / T gene polymorphic loci genotype frequency distribution. The data were analyzed using SPSS11.5 software package (SPSS Company, Chicago, Illinois, USA). The age difference of the case group and control group t test. Hardy-Weinberg equilibrium, compare that genotype frequencies observed and expected values ??and the chi-square test line. The genotype distribution between the two groups was used to compare the list of rows × chi-square test. Non-conditional logistic regression to calculate the ratio of the relative risk (odds ratio, OR) and 95% confidence interval (confidence interval, CI). EH software and the 2LD software analysis VEGF gene promoter region-460C / T, -1 154 G / A polymorphic loci haplotype frequencies and linkage disequilibrium in allele. P lt; 0.05 as a significant difference between the standards. Results: 1 healthy control group VEGF gene promoter-1154G / A,-460C / T genotype frequencies of two polymorphic sites were in accordance with Hardy-Weinberg balance (P> 0.05). In ovarian cancer and control groups, the promoter region of the VEGF gene-1154G / A polymorphism frequency of the three genotypes (A / A, G / A, G / G) are 1.7%, 17.8%, 80.5 % and 2.3%, 26.1%, 71.6%, and the difference was statistically significant (P = 0.037); ovarian cancer and control groups (G, A) allele frequencies are 89.4%, 10.6 % and 84.7%, 15.3%, the difference was statistically significant (P = 0.013); compared with G / AA / A genotype, carrying the G / G genotype may significantly increase the risk of epithelial ovarian cancer ( OR = 1.64,95% CI = 1.12 ~ 2.39). VEGF gene promoter region-460C / T polymorphism C, T allele frequency in ovarian cancer and control groups were 19.3%, 21.3% and 80.7%, 78.7%, and the two groups no significant difference (P> 0.05); C / C, C / T, T / T genotype frequency in ovarian cancer and control groups were 4.0%, 30.7%, 65.3% and 5.6%, 31.4%, 63%, two sets of phase no statistically significant difference (P> 0.05); compared with C / TT / T genotype, T / T genotype may be related to the risk of ovarian cancer, the OR value 1.11 (95% CI = 0.79 to 1.54) . VEGF gene promoter region-1154G / A and-460C / T SNPs loci in linkage disequilibrium (D '= 0.426). Conclusion: VEGF gene promoter region-1154G / A polymorphism may be associated with the risk of epithelial ovarian cancer significantly correlated, that is carrying the G / G genotype may significantly increase the risk of epithelial ovarian cancer. 2 VEGF gene promoter region-460C / T single nucleotide polymorphism may be nothing to do with the risk of epithelial ovarian cancer. VEGF gene promoter region-1154G / A and-460C / T SNPs loci linkage disequilibrium.
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