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Objective To observe neuronal nitric oxide synthase ( nNOS ) , inducible nitric oxide synthase (iNOS) after birth cynomolgus monkey (Macaca fascicularis) expression in testis development process characteristics to explore both testicular development and spermatogenesis occurred mature role . 28 male cynomolgus monkeys Materials and methods according to the degree of development of testicular seminiferous tubules divided into five developmental stages : newborn monkey period 4 4 Child monkey pre youngster monkey late 6 , youthful monkey 6 adult monkeys period 8 . Testicular tissue were acquired with Bouin's solution fixed 4-12 hours , embedded in paraffin the slice thickness 6μM, attached to poly-L-lysine -treated slide , slice dewaxing routine HE staining . Eli VisionTM plus immunohistochemical techniques detect cynomolgus monkeys from newborn monkey the different developmental stages to the adult monkey period nNOS, iNOS expression . Pathological image analysis system to calculate the various developmental stages of testicular positive cells , positive cell rate of testicular developmental stages using SPSS13.0 software for statistical analysis . NNOS results in newborn monkeys \u0026 Tong monkey pre free expression, the late youngster monkey , youth Monkey period , adult monkey spermatogonia of the cytoplasm , the cytoplasm of primary spermatocytes expression . After three developmental stages of testicular cells nNOS positive rate in a statistically significant difference ( P lt; 0.01 ) , the positive rate is the highest in youth monkey , followed by the late Tong monkey , the adult monkey period less , newborn monkey period The pre and Tong monkey free expression . iNOS in newborn monkeys , Tong monkey pre free expression, late in the youngster monkey , youth Monkey period , the expression of the interstitial cells of the adult monkey , three positive expression rate was statistically significant difference ( P lt ; 0.01 ) , the highest youth monkey period the Second a late youngster monkey , adult monkey duration of at least . Conclusions in different developmental stages after birth , in the cynomolgus monkey testis nNOS, iNOS expression occurred dynamic changes prompted both testicular development and spermatogenesis , mature important .
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