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Cnidium Umbelliferae snake bed is the fruit of the plant Cnidium Cnidium monnieri (L.) Cusson, one of the drugs commonly used in Chinese medicine clinical, wet kidney impotence, dampness, the effect of the insecticide. The Cnidium widely distributed in most parts of China, due to geographical location and ecological environment of different the intraspecific chemical composition larger variation. Now generally think the the coumarin compounds Cnidium main active ingredient, comprehensive development and utilization of resources of medicinal plants, looking for a new drug with the resources of Cnidium Cnidium, the subject for the study, and the establishment of a modern chromatography them the coumarin component characterization methods. RP-HPLC, small diameter column high-performance liquid chromatography, β-cyclodextrin-modified micellar electrokinetic capillary chromatography, pressurized capillary electrochromatography method for studying technical, the Cnidium herbs coumarin compounds fingerprint characterization and quality control, modern research Cnidium herbal quality control template of research methods and technology platform. 1. RP-HPLC measured determination snake snake snake snake bed bed bed Chuangzi sub-sub-drugs herbs savory savory Peas Peas the Susu elements into the composition by reversed-phase high-performance liquid chromatography (RP-HPLC) method 12 batches of different sources Cnidium herbs characteristic component separation analysis, to evaluate the quality characteristics of component spectrum. Octadecylsilane bonded to the silica gel column DiamonsilTM C18 column (150 mm × 4.6 mm, 5 μm), mobile phase acetonitrile -10 mM ammonium acetate (adjusted to pH 4.0 with acetic acid) (50:50) ; The detection wavelength was 320 nm. Stability as an internal standard, bergapten Europe decursin, 2'-acetyl archangelicin, the European mountain apigenin O-acetyl Columbia lactone regression equations were Y = 0.0279X-0.0203 Y = 0.0197X 0.041 Y = 0.0251X 0.032, Y = 0.0368X 0.0252, Y = 0.0325X 0.0228, the linear range 4μg/mL to 100μg / mL; the regression equation osthole Y = 0.0472X-0.0539, linear range 10μg/mL ~ 300μg / mL; average recovery and RSD were 98.1%, 96.2%, 96.4%, 101.9%, 102.4%, 98.4% and 4.58%, 5.73%, 3.58%, 4.89%, 5.85%, 4.51%. The method is simple, fast, good recoveries and reproducibility applied to optimize the separation and bergapten coumarins in Cnidium herbs, Europe decursin, osthole 2'-acetyl archangelicin, European mountain celery The hormone and O-acetyl Columbia lactone quantitative analysis, and to the herbs Cnidium quality control and authentic research to provide a simple method. 2 small diameter column RP-HHPP LC Determination of coumarin ingredients in Cnidium herbs the basis of the previous chapter, the conventional column to the small diameter columns, the establishment of a fast, simple, high reproducibility fine diameter column HPLC method for simultaneous determination of coumarin compounds in 12 batches of different sources Cnidium herbs bergapten Europe decursin osthole 2'-acetyl archangelicin, European mountain apigenin and O-acetyl Colombia lactone content. Phenomenex? C18 column (150 mm × 2.1 mm, 5μm); mobile phase: acetonitrile -10 mM ammonium acetate (adjusted with acetic acid to pH 4.0) (50:50); flow rate of 0.3 mL / min; ultraviolet detection wavelength of 320 nm; injection volume of 20 μL. The experimental results show that the method is sensitive, accurate, reproducible and suitable for content determination. Micellar electrokinetic capillary chromatography coumarins in Cnidium herbs established method of β-cyclodextrin-modified micellar electrokinetic capillary chromatography separation and determination the Cnidium herbs in coumarin compounds. Done a systematic study of the internal Choose a pH buffer concentration, buffer composition and concentration of influencing factors. Using uncoated fused silica capillary column 50.2 cm × 75μm id, effective length of 40 cm, the running buffer of 40 mM borate buffer -25 mm SDS-10 mM hydroxypropyl cyclodextrin (pH 9.5) and 20% methanol The column temperature was 25 ° C, operating voltage of 30 kV () → (-) pressure injection 1 psi × 5 s, detection wavelength of 210 nm. Simultaneous determination of different purchase to Cnidium six coumarin constituents in herbs bergapten Europe decursin, osthole 2'-acetyl archangelicin, the European mountain apigenin and O-acetyl Columbia lactone content. Separation and analysis results Cnidium herbs with certain applications in the field of micro-separation prompted role, provide a favorable basis for the quality control of the production process. Pressure capillary chromatographic separation pressurized capillary electrochromatography (pCEC) determination of the Cnidium herbs coumarin ingredients to establish the herbs Cnidium quality control of micro-column analysis methods. First, based on the separation of the core pressure capillary electro chromatography capillary column, the two ends of the conventional sintered plug packed column inlet end for the open string and the outlet end of capillary chromatography column packed with the overall plunger sub repeatability and feasibility of the preparation method of study and research. Second, the self-made new capillary column coumarins in Cnidium separation and acetonitrile ratio of buffer ionic strength, pH, and operating voltage conditions optimized electrical optimized chromatographic conditions in within 15 min separation of six coumarins in Cnidium medicinal compounds the (osthole, bergapten imperatorin, the European Mountain apigenin, 2'-acetyl archangelicin O-Columbia lactone) and linearity, precision, stability, sample recovery, a more comprehensive study. The experimental results show that the method is sensitive, accurate, reproducible and suitable for content determination. The results pressurized capillary electrochromatography separation and analysis of traditional Chinese medicine as a favorable analysis technology to provide a reliable basis.
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