Dissertation > Excellent graduate degree dissertation topics show

Inter- electrospray ionization mass spectrometry peptide molecular interactions

Author: PuLing
Tutor: DingChuanFan
School: Fudan University
Course: Chemistry
Keywords: Electrospray ionization mass spectrometry Intermolecular interactions Mass Spectrometry Non-covalent complexes Hydrogen bonds Ion peak Protein molecules Non-covalent Soft ionization technique Mass analyzer Mass spectrometry data Polypeptide chain Side chain Protein Mass Spectrometry Three-dimensional structure Protein Structure Hydrophobic interaction The peak intensity Chain sequence
CLC: O629.72
Type: Master's thesis
Year: 2011
Downloads: 88
Quote: 0
Read: Download Dissertation

Abstract


Protein is very important to biological life. It is the major component of life and major life activities actor. The physiological functions of protein are determined by the proteins’structure. Protein is composed of polypeptide chains that are formed by 20 different amino acids. It is useful to learn the three-dimensional structure of protein through studying the non-covalent interaction between the peptides. This thesis consists of three phases work:The first stage of the study is that use the ESI-MS to study the non-covalent interaction between peptides. The 16 pairs 5-peptides in my study is based on the X-ray diffraction data of the protein which distance between the peptides is less than the threshold value, and we assume that two peptides interact each other. To check this hypothesis, we find the company to synthetic these peptides and study if they can form complex by mass spectrometry method.The second step based on the results of the first stage experiment. We select eight peptides and studied the interaction of the peptides, then calculated the equilibrium constant to compare the interaction strength.Based on the first and second steps, we tried to change the length of the peptide and the amino acid sequence of the peptides to study the effect of peptide length, hydrogen bond, hydrophobic interactions and sulfhydryl group to the interaction of peptides.The results support that peptides can interact each other when distant between the two peptides is less than the threshold value. Lengthened the 5-peptide to 9-peptide, the interactions between 5-peptide and 9-peptide were no significant change. It is mainly because the synergies between non-covalent interactions. Changed the sequence peptide, the non-covalent interaction had significant changes. Hydrogen bond and hydrophobic play important roles in non-covalent interaction between peptides,which strengthen the interaction. And also thiol side chain substitution had significantly effected the interactions between peptides, thiol side chain impede the non-covalent interaction.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Recovery Whey Soy Protein by Microfiltration and Development of Whey Powder,TQ936.2
  3. Research on Disease Related miRNA Mining Based on Protein-Protein Interaction Network,R341
  4. The Research and Implementation of Protein Classification Algorithm on the Basic of String Kernel,TP301.6
  5. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  6. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  7. The Study of Color Change and Control Technology for Oyster Protein Beverage,TS254.4
  8. Discovery and Biological Activities of Natural Michael Addition Acceptors,R284
  9. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  10. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  11. Metabonomics Study on the Usnic Acid and Erthromycin Estolate-induced Liver Toxicity by GC-MS,R965
  12. Impact on Drosophila Brain Developement Through the Overexpression and Loss of Function of Gsα,Q75
  13. Study on Deodorization Techniques of Oyster Protein Beverage,TS254.4
  14. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  15. Study on Preparation and Properties of Protein Isolates from Tilapia,TS254.4
  16. Study of Triptolide Induced Hepato-toxicology by MS-based Metabonomics Approaches,R285
  17. Expression and Purification of Influenza a Virus M2 Protein and Detection of Its Antigenicity,R392
  18. Broiler Responses to Reduced-protein Diets Suppleme Nted with Synthetic Amino Acids and Small Peptides,S831.5
  19. The Preparation of Hpv58e7 Protein by GST-tagged Prokaryotic Expression System,R392
  20. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  21. Comparative Study on Reproductive Biological Characteristics of Helicoverpa Armigera and Helicoverpa Assulta (Lepidoptera:Nuctuidae),S433

CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Natural compounds > α-amino acids,peptides, proteins, nucleic acids > Peptide
© 2012 www.DissertationTopic.Net  Mobile