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Effect of Gentamicin on ET-A Expression in Cochlear Stria Vascularis and the Protection of Sodium Ferulate to Gentamicin-induced Ototoxicity

Author: ZhuRongHua
Tutor: WangHaiBo
School: Shandong University
Course: Otorhinolaryngology
Keywords: Gentamicin Ototoxicity Endothelin Sodium ferulate
CLC: R764.35
Type: Master's thesis
Year: 2010
Downloads: 29
Quote: 0
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Abstract


Background and Objectives: Gentamicin is a clinical application of the more common aminoglycoside ototoxicity caused by sensorineural deafness is more common, but its ototoxic mechanism is unclear, there is no ideal The method for prevention. This project by constructing the gentamicin-induced deafness rat model, the observed changes gentamicin - induced ear morphology of cochlea hair cells in the process of toxicity, cochlear outer side wall of the vascular pattern pathological changes of the capillary network and ET-A expression changes, the possible mechanism of ototoxicity, and further explore the protective effect of sodium ferulate on ototoxicity, which seeks to reduce ototoxicity. Methods: 32 Wistar rats were randomly divided into three groups: control group (n = 8), gentamicin group (n = 13, 150 mg / kg intramuscularly 7 days), gentamicin (150 mg / kg intramuscularly 7 days) and ferulic acid sodium (50 mg / kg intramuscularly 7 days) combined treatment group (n = 11), were established animal model. Application brainstem detected brainstem auditory evoked potential threshold of hearing (auditory brainstem response, ABR) changes in rats in each group before and after treatment; basement membrane stretched stretched and vascular pattern observed in each group of hair cells and microcirculation morphological changes; immunohistochemical SP method was used to observe the situation of each group of drugs on cochlear outer sidewall endothelin A (ET-A) receptor expression. SPSS13.0 statistical software ABR change group comparison and each group ET-A expression changes are used the correction t test was used for statistical analysis. Results: intramuscular injection of gentamicin rats ABR hearing threshold than the control group significantly increased (P lt; ABR change of 0.05) and gentamicin group was significantly higher than the combined treatment group ABR hearing threshold changes, a statistically significant difference (P <0.05); combined treatment group ABR changes slightly higher than ABR changes, P gt; 0.05, the difference was not statistically significant (P> 0.05). The gentamicin group hair cells arranged clutter, the apparent lack of vascular pattern capillary network showed a contraction ischemia; the combination group hair cells derangement, scattered missing, the vascular pattern capillary network no significant contraction ischemic changes. ET-A is mainly distributed in the vascular pattern intermediate cells and microvascular cells around the wall, the control group and the combination group was weakly positive (), gentamicin group was strongly positive (). Conclusions: 1. Gentamicin intramuscular injection can significantly improve the rat's hearing threshold, causing significant hearing loss, the SF can effectively antagonize gentamicin ototoxicity in rats, protection rats hearing. Gentamicin can cause vascular pattern of necrosis and apoptosis cochlear outer wall of the cochlea hair cells capillary network strong contraction, suggesting that gentamicin can cause the cochlear outer side wall of the microcirculation, suggesting that the Lateral Cochlear microcirculation participate celebrate large neomycin ototoxicity; of sodium ferulate gentamicin combination can effectively protect hair cells to reduce injury, improve the microcirculation of cochlear outer sidewall. 3 the cochlear outer sidewall ET-A may be involved in the process of gentamicin induced ototoxicity, ferulic acid sodium may antagonize gentamicin side effects by inhibiting ET-A role. 4 ototoxicity caused by cochlear outer sidewall microcirculation and increased ET-A expression may provide a theoretical basis for the clear aminoglycosides mechanism of drug-induced ototoxicity may be effective protective effect of sodium ferulate prevention and treatment of clinical aminoglycoside-induced ototoxic provide new therapeutic strategies.

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CLC: > Medicine, health > Otorhinolaryngology > Otology,ear disease > Inner ear disease > Cochlear disease
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