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The first part of the inflammatory bowel disease The serum autoantibody spectrum of research background of inflammatory bowel disease (IBD) is a cause is not yet very clear chronic non-specific inflammatory bowel disease, including ulcerative colitis (UC) and Crohn's disease (CD), the performance of bleeding abdominal pain, diarrhea, fistula, anal lesions and varying degrees of systemic symptoms. IBD diagnosis relies on clinical manifestations, colonoscopy, imaging studies, and biopsy. At home and abroad widely reported anti-Saccharomyces cerevisiae cell antibodies (ASCA). Joint detection of anti-neutrophil cytoplasmic antibody (ANCA). Resistant intestinal goblet cell antibodies (GBA) and anti-the pancreatic acinar antibody (PAB) for the diagnosis and differential The diagnosis of UC and CD are important, and can guide the classification and treatment of IBD. Objective To investigate the combined detection of serum ASCA.ANCA.GBA and PAB IBD diagnosis and differential diagnosis. Research methods using ELISA method and indirect immune fluorescence method respectively determination of 159 cases of IBD patients, including the UC 97 Li, the CD 62 Li, 167 cases the main complaint is abdominal pain, diarrhea, and except for IBD patients and 25 healthy controls by serum IgG type and the the IgA type ASCA and ANCA. using indirect immunofluorescence were measured in 20 patients with UC, 20 patients with CD, 20 cases of patients with intestinal diseases, and 10 healthy control group, serum GBA and PAB level. Results ASCA-IgA/IgG positive rate in the CD group, UC group, the disease control group and normal control group were 43.5%, 14.4%, 29.3% and 0, the positive rate in the CD group was significantly higher than the UC group and disease control group (x2 = 16.76, x2 = 4.12; P lt; 0.001, P = 0.042); ANCA-in the above groups, the positive rate, respectively, to 8.1%, 56.7%, 4.8% and 0 UC group positive rate significantly with higher than the CD group and the disease control group (x2 = 38.08, x2 = 90.47; P lt; 0.001, P lt; 0.001); ASCA / ANCA-a combination of diagnostic CD sensitivity, specificity, positive predictive value of 40.3%, 93.8% and 80.6%; ANCA / ASCA-combination of diagnostic UC sensitivity, specificity and positive predictive value of 48.5%, 98.4% and 97.9%, respectively; ASCA in CD patients did not undergo surgical treatment and surgical treatment positive rate difference was statistically significant (P = -0.03); ASCA-IgA positive rate in CD patients (33.9%) higher than the ASCA-IgG (27.4%), but no significant difference (P = 0.481); ASCA The inconsistent-IgA and ASCA-IgG-positive patients the percentage was 27.4%. GBA positive rate in the UC, CD, disease control and healthy control group were 30%, 65%, 10% and 0% of CD patients, the positive rate was significantly higher than the control of disease and the healthy control group (P lt; 0.05) UC group, no significant difference (P gt; 0.05). PAB UC, CD, disease control and the healthy control group, the positive rate of 5%, 25%, 0% and 0%, the positive rate of four groups no significant difference (P gt; 0.05). The GBA / ANCA-diagnosis of CD sensitivity, specificity, positive predictive value, and negative predictive values ??were 45%, 95%, 90% and 63.3%, respectively. ANCA-conclusion, ASCA and GBA positive favor the diagnosis of inflammatory bowel disease (IBD) is not sensitive screen; the ANCA-ASCA and GBA joint detection as the differential diagnosis of UC and CD, is one of the differential diagnosis of IBD non-traumatic. Simultaneous detection of IgA and IgG ASCA improve the diagnostic sensitivity of the CD. Chinese population ASCA positive may be related to the surgery may be a potential secondary indicators to guide surgery. The second part of the human myeloid cells the triggering receptor -1 (TREM-1) mRNA expression and clinical significance of background in ulcerative colitis ulcerative colitis (ulcerative colitis, UC) is a chronic, non-specific colitis, severe cases, ulcers, lesions mainly involving the colonic mucosa and submucosa; range start from the distal colon, retrograde proximal development, even involving the entire colon and terminal ileum was continuous distribution. Understand the extent and scope of UC inflammatory response helps to choose a reasonable treatment plan. Colonoscopy and biopsy is the main way to determine disease activity, but because of its high cost, pain, poor patient compliance repeated examination. Myeloid cells triggered receptor -1 (triggering receptor on myeloid cells-1, TREM-1) is found in recent years, a new indicator for diagnosis of early inflammatory response, is activated receptor of the immunoglobulin superfamily, selectively expressed in blood neutrophils and monocytes. Objective To compare the TREM-1 expression levels in PBMCs of patients with active UC, compared with the healthy population, and to explore the TREM-1 in the UC disease activity relationship, as well as its role in pathogenesis. Research methods by reverse transcription real-time quantitative polymerase chain reaction (FQ-RT-PCR) method to detect 38 cases, 70 patients with UC (active patients to alleviate period 32 patients) and 20 healthy patients (control group) TREM-1 mRNA expression levels in peripheral blood mononuclear cells (PBMCs) and content using the relative quantification of the level of the ΔCt value to compare the gene expression levels, and compare TREM-1 mRNA expression levels of inflammatory markers ESR and CRP correlation. The results, patients with active UC PBMCs TREM-1 mRNA expression levels (4.19 ± 1.86) is much higher than UC patients in remission (5.29 ± 1.71) and normal controls (5.19 ± 1.04) (ΔCt gene expression was negatively correlated ΔCt lower, the higher the amount of gene expression), UC remission in patients with (5.29 ± 1.71) and normal control group (5.19 ± 1.04) was no significant difference; TREM-1 mRNA expression and ESR in patients with UC active phase ( P = 0.003), whereas serum CRP levels were not related (P = -0.055). Conclusion TREM-1 mRNA expression is significantly associated with UC disease activity, TREM-1 is indeed involved in the inflammatory process of UC. TREM-1 mRNA expression levels correlated with ESR and serum CRP levels were not related.
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