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Objective: To investigate TGF-β1 on cultured bone marrow mesenchymal stem cells (BMSCs) VEGF expression methods : ( 1 ) SD rats were cultured in vitro bone marrow mesenchymal stem cells, cell growth was observed daily status and identification. ( 2 ) Different concentrations of TGF-β1 at different points in time acts on BMSCs, the supernatant was observed by ELISA VEGF protein expression , RT-PCR expression of VEGF mRNA was observed . (3) TGF-β1 at 1 hour, 2 hours, 3 hours pretreatment BMSCs, Akt phosphorylation was observed , and the expression of phosphorylated Erk . (4) Akt channel blocker LY294002, Erk channel blocker U0126 pretreated for 1 hour , TGF-β1 acts on BMSCs24 hours , respectively, with ELISA and RT-PCR was observed VEGF protein and mRNA expression . (5) LY294002 and U0126 pretreated for 1 hour , TGF-β1 acts on BMSCs, were observed for 1 hour, 2 hours , 3 hours , phosphorylated Akt and phosphorylated Erk1 / 2 in the expression . Results: ( 1 ) successfully cultured rat BMSCs, flow cytometry cell surface markers CD45 (-) CD90 (), the cells used in this experiment for the first 3-5 cells . (2) TGF-β1 can be a time - concentration - dependent promote BMSCs to express VEGF, and the protein and mRNA level verification. (3) TGF-β1 expression of VEGF to promote the role of BMSCs can be Akt channel specific blocker Ly 294002 antagonized , can also be of ERK1 / 2 specific inhibitor U0126 blocked by that Akt and ERK1 / 2 channel may be involved in this process. Conclusion : TGF-β1 in vitro was time to - concentration - dependent expression of rat BMSCs VEGF, and this may be through promoting secretion of Akt and ERK1 / 2 activation channel to achieve.
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