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Isolation, Culture and Plurilineage Induction of the Thawed Human Mesenchymal Stem Cells

Author: XiangYing
Tutor: WangJinFu
School: Zhejiang University
Course: Cell Biology
Keywords: Bone marrow mesenchymal stem cells Culture Differentiation Chondrocytes Fat cells Neurons
CLC: R329
Type: Master's thesis
Year: 2005
Downloads: 165
Quote: 0
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Abstract


Addition to containing hematopoietic stem cell differentiation and development into various blood cells in the bone marrow, can produce non-hematopoietic tissue contains mesenchymal stem cells (Mesenchymal stem cells, MSCs). MSCs on the one hand to support the survival, growth and differentiation of hematopoietic stem cells in the bone marrow. On the other hand, many in vitro experiments show, they are a multi-differentiation potential of cells, has a strong ability to self-replicate, can differentiate into a variety of tissues and cells of mesodermal origin (such as specific conditions: across mesoderm cells (such as neurons) can differentiate into osteoblasts, chondrocytes, muscle cells, etc.), and even. MSCs have easily obtained, there is no immune rejection, cell expansion and easy easy clinical application characteristics, is an important tissue engineering seed cells. MSCs have unique cell proliferation split mode so that the exogenous gene to be easily introduced and expression, so that it is expected to become a potential cell therapy and gene therapy in human medicine to a target cell, tissue outside in a variety of hematopoietic defects diseases, degenerative diseases and genetic diseases, cell therapy and gene therapy has an important application prospect. MSCs in vitro after long-term culture will encounter some unfavorable factors, such as gene flow, cellular senescence, differentiation and phenotypic instability, pollution or incubator failure, which would affect the application of MSCs. Therefore, the cryopreserved MSCs in a timely manner is an important means of cells seed reserves in the future. However, cryopreservation how the expansion and differentiation of MSCs, worthy of further study. Vitro experiments on adult bone marrow mesenchymal stem cells (human mesenchyma] stem cells, hMSCs) isolated, cultured and initial cells induced to differentiate, and observe their growth, and aims to establish a separate culture hMSCs and effective way to induce cartilage cells, fat cells and nerve cells into hMSCs as a cell source for tissue engineering to provide experimental evidence. Recovery and passaged for 15 generations for the detection of cryopreserved hMSCs is still multi-differentiation potential to the cartilage, fat, and neurons through adhesion method obtained from the bone marrow hMSCs and frozen on the basis of the recovery cells were passaged for 15 generations, and then turn chondrogenic culture liquid under conditions of fat cells and neuron cells, its morphological changes were observed in the corresponding role in the induction of liquid collagen II, triolein glycerides, Nestin (Nestin) and neuron-specific enolase (neuron-specific enolase, NSE) expression, which studies the cryopreserved cell expansion and differentiation potential. The research results show that the cryopreserved hMSCs still in recovery after a single feature, homogeneous cell populations. Its

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CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
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