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Objective: To explore the angiotensin II -1 receptor (AT1-R) gene A1166 / C polymorphism with obstructive sleep apnea hypopnea syndrome (obstructive sleep apnea-hypopnea syndrome, OSAHS) and sleep apnea hypopnea syndrome with hypertension (OSAHS and Hypertension) correlation. Aimed at looking the simple of OSAHS \u0026 OSAHAHT genotypes, and to study the role of this gene polymorphism in the OSAHS and OSAHAHT incidence and progression. METHODS: We randomly select 65 cases of OSAHS patients (38 males, 27 females), all patients had nighttime sleep snoring, history of apnea and daytime sleepiness, and confirmed by polysomnography (polysomnography, PSG) monitoring, OSAHS ≥ 5 times / h. Refers to the nose and mouth breathing airflow during sleep apnea hypopnea stop 10s, more basic level of intensity of respiratory airflow during sleep accompanied by reduced by more than 50% compared with baseline oxygen saturation levels ≥ 4%. Including 31 cases of simple, uncomplicated patients with OSAHS, age 32, ~~ 71 (47.5 ± 10.3) years, BMI 23.04 to 33.61 (28.68 ± 3.16) kg/m2. 34 patients OSAHAHT age 32 to 76 (52.55 ± 12.74) years, BMI 26.12 ~ 39.79 (29.48 ± 3.89) kg/m2, in line with OSAHS diagnostic criteria, and the Chinese Hypertension Prevention Guide \diagnostic criteria for blood pressure, hypertension occurs later than with OSAHS and to exclude of secondary nephrogenic endocrine hypertension reasons; control group of 70 patients (40 males, 30 females cases), aged 26 to 55 years old (47.65 ± 8.07), body mass index, 21.67 to 29.76 (27.13 ± 2.14) kg/m2, after history taking and line Stardust portable sleep monitors screening examination to exclude OSAHS. Age and body mass index in the three groups were compared no statistically significant differences (all p gt; 0.05), and except for the confounding factors of secondary hypertension and ischemic heart disease. All selected candidates in sleep apnea monitoring end the morning woke Blood samples from 5 ml of 5 minutes, respectively, application of phenol - chloroform extraction of DNA and then the polymerase chain reaction (PCR) to amplify DNA fragments, using restriction endonuclease enzyme cut, electrophoresis genotyping assay the AT1R gene A1166 / C polymorphism. The three groups of genotypes AA, AC, CC compared using chi-square test the simple OSAHS with OSAHAHT of patients with sleep apnea monitoring indicators compared using t test. Results: 1 the AT1R gene AA in the normal population, AC, CC-frequency distribution were 88.2%, 10.3%, 1.5%, OSAHS patients with gene frequencies were 72.3%, 26.3%, 1.4% of patients with OSAHS and control group compare their constitutes a significant difference (χ 2 = 5.733, P lt; 0.05). A and C allele frequencies in the normal population were 93% and 7%, OSAHS patients with allele frequencies were 85% and 15% of the two groups have a significant difference (χ2 = 4.861 P lt; 0.05). The frequency of of OSAHS patients alone allele A and C, respectively, 62% and 38%, respectively. OSAHS patients with hypertension were 80% and 20%, respectively. Its distribution have significant differences. (Χ2 = 5.355 P lt; 0.05) 2.OSAHAHT patient group alone OSAHS patients, OSAHAHT patient groups AT1R gene AA, AC, CC-frequency distribution were 71%, 29%, 0%, pure OSAHS patients group the AT1R gene AA, AC, CC type distribution frequency were 74%, 23%, 3%, OSAHAHT patient group alone patients with OSAHS group constitute no significant difference (χ2 = 0.105, P gt; 0.05). Group A and C of OSAHAHT patients with allele frequencies were 80% and 20%, simple the OSAHS patient group A and C allele frequencies of 62% and 38%, respectively, the OSAHAHT patient group alone OSAHS patients group constituting a significant difference (χ 2 = 5.355, P lt; 0.05). And OSAHAHT patient group in comparison with simple OSAHS patient group, wherein A allele frequency was significantly higher than the C allele frequencies. 3 simple OSAHS patients sleep apnea monitoring indicators of the OSAHAHT patients with AHI, average SaO2, mean apnea time were statistically significant (t = 0.7567,0.54,2.2615; p lt; 0.05, p lt; 0.05 p lt; 0.05), while the longest apnea time, minimum oxygen saturation, SaO2 lt; 90% of the time, no statistically significant difference between the two groups (all p gt; 0.05). 4.AA, AC, CC three different genotypes OSAHS patients with sleep apnea monitoring indicators compared the three genotypes AHI, the average apnea, SaO2 lt; 90% of the time, the lowest SaO2 and rapid eye movement sleep accounts the percentage of total sleep time (REM / TST) were statistically significant (p lt; 0.05). Three different genotypes of sleep-disordered breathing longest apnea was no significant difference (p gt; 0.05). Conclusion: 1.OSAHS incidence AT1R gene A / C polymorphism is associated. AC CC genotype frequencies a simple OSAHS and OSAHAHT of patients was significantly higher than the normal control group. And 1166C allele frequency was significantly higher than the normal control group. Simplex OSAHS with OSAHAHT patients compared allele frequencies of A and C have significant differences. The 2.AC and the CC genotype of OSAHS patients with sleep apnea hypopnea index (AHI), the average duration of apnea, the lowest oxygen saturation with the AA genotype difference was statistically significant. OSAHS pathogenesis of AT1R gene A / C polymorphism is associated, genotype AC and allele C OSAHS pathogenesis risk factors.
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