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Objective: To evaluate for perinatal hypoxic - ischemic encephalopathy and hypoxic-ischemic brain damage caused by cerebral palsy and HIF-1α the gene C1772T/G1790A the single nucleotide polymorphism , early intervention reduce the incidence of hypoxic-ischemic encephalopathy and hypoxic-ischemic encephalopathy due to cerebral palsy . Find a better theoretical basis for neonatal hypoxic -ischemic encephalopathy prevention . Subjects and methods : 1 . Divided into three groups : group A : 78 cases of neonatal hypoxic- ischemic encephalopathy , HIE clinical manifestations degree of grade , the mild HIE children with 38 cases of children with moderate HIE 26 cases of severe HIE children 14 cases ; B group : 100 cases of normal controls ; C group: neonatal hypoxic-ischemic encephalopathy caused 92 cases of children with cerebral palsy based on past HIE clinical manifestations classification , mild HIE children 16 cases , 45 cases of children with moderate HIE severe 31 cases of HIE ; 2 . was extracted from peripheral venous blood of patients and normal newborns were extracted genomic DNA reference kit ; polymerase chain reaction (PCR) amplification of target gene the 346bp 4.PCR product was digested and electrophoresis . Results: 1.A , B, C three groups of gestational age , birth weight , sex differences and feeding patterns no significant differences in sex ( P gt ; 0.05) ; 2.A group of HIF-1α gene 1772CT genotype share more ( 30.77% ) , B , C group HIF-1α gene 1772CT genotype share less, respectively, 18 % , 18.57% , three groups A , B , C, HIF-1α gene C1772T / genotype distribution was significantly different (P lt; 0.05 ) , group A group of mild, moderate , and severe the HIE in children comparing expression of HIF - 1α gene C1772T / genotype distribution no statistically significant difference ( P gt ; 0.05) , C group with mild , moderate , severe HIE the children compare HIF-1α gene C1772T / genotype distribution there is a significant difference ( P lt; 0.05 ) ; 3.A group B HIF-1α gene G1790A genotype distribution does not meet the Hardy-Weinberg equilibrium test ( P lt ; 0.05) groups A, B, C HIF-1α gene G1790A gene polymorphism is not to compare . Conclusion: The incidence and hypoxic-ischemic brain damage caused by cerebral palsy and HIF-1α gene C1772T single nucleotide polymorphisms hypoxic-ischemic encephalopathy associated .
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