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Rat IL-6 gene eukaryotic expression plasmid

Author: HanMingGuo
Tutor: ZhaoXueXin；KuReXi·YuNuSi
School: Xinjiang Medical University
Course: Biochemistry and Molecular Biology
Keywords: IL-6 RT pcDNA3.1/CT-GFP-TOPO
CLC: Q78
Type: Master's thesis
Year: 2006
Downloads: 34
Quote: 0
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Abstract


Objective: to regulate gene expression for the application of RNAi technology, build rat interleukin 6 gene (interleukin - 6, IL - 6) eukaryotic expression plasmid. Methods: total RNA extracted from rats colon, by rt-pcr (reverse transcription) get cDNA gene, gene fragments amplified by PCR from purpose, connect it into pcDNA3.1 / CT - GFP - TOPO carrier, the identification of recombinant, transfection COS 7 cells. Results: the successful build the purpose gene recombinant, sequencing of nucleotide sequence is correct, according to a report in COS 7 cells after transfection visible green fluorescence in fluorescence microscope. Conclusion: rat eukaryotic expression plasmid pcDNA3.1 / CT - GFP - successful build of TOPO/IL - 6, as the application of RNAi technology regulation of il-6 gene expression, provides the experimental basis for the study of IL - 6 provide experiment platform with ulcerative colitis.

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